What are chromatography and colourimetry in chemistry?
Chromatography: is an important technique in chemistry. This technique used to purify a sample and also identify compounds in an unknown mixture. Chromatography is an analytical technique for the purification and separation of substances. It is also found useful for the fractionation of complex mixture, separation of compounds and isolation of unstable substances.
Column chromatography is used in pharmacy to determine the amount of each substance found in new products. It also uses in hospitals to detect blood or alcohol levels in patient’s bloodstream. In forensic science, chromatography plays an important role. It is used to compare the sample found at a crime scene with the sample taken from a suspect. It has environmental usage as well some agencies use chromatography technique to determine the level of pollution in the water supply.
Colourimetry: is a technique used in biomedical investigations it involves the quantitative estimation of colours. The intensity of the colour is directly proportional to the concentration of the compound.
Colourimetric technique has a wide range of usage in science. In biology, it uses to determine the growth of bacterial or yeast culture. As the culture growth, the medium becomes more cloudy and absorbs more light. The amount of light absorbed depends on the amount of solute in a solution. The higher the concentration, the more light will absorb.