How the concentration of the agarose influences the study of the DNA?

Asked on 10.12.2018 in All Questions.
Add Comment

Tutor's Answer

(Top Tutor) Studyfaq Tutor
Agarose gel is a polysaccharide which acts a sieve to “catch” the molecules as they are transported through the matrix by the electrical current applied. The pore size of the agarose gel impacts the size of the DNA that can be sieved because only molecules of a certain size will be able to fit through certain pores. Smaller molecules will be able to pass through more easily than larger molecules which will have to contest with the meshwork of the gel made up of long chains of interlinked sugars. Thus, molecules of DNA are separated according to size as smaller molecules will be able to travel further. Different...
Completed Work