What are the methods to use noradrenaline?

Asked on 21.12.2018 in All Questions.
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Drug solutions were prepared by adding each pharmacological agent to 40 mL of modified Kreb’s solution. Desipramine was prepared as a one mM solution, and noradrenaline was 1x 10-5 M. All other drug solutions were made to have a concentration of 1x 10-6 M. The stock solutions were placed in a 35c water bath. Brains were obtained from rats and placed in a chilled, modified Kreb’s solution. The brain was cut into round slices with approximately a 0.5 mm thickness and a 4 mm diameter, and the masses of these slices were recorded (this was done for us by the lecturer present). Next, four nylon mesh bags were constructed by sewing together two 15 x 15 mm squares of the material....
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