What is the process of data analysis?

Asked on 16.12.2018 in All Questions.
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The MMLV reverse transcriptase works just like the reverse transcriptase of the second strand synthesis method, but it adds on a set of nucleotides onto the 3’ end of the cDNA it just synthesized, which then becomes the anchor for the template switching oligonucleotide. The presence of the template switching oligo causes the reverse transcriptase to switch to the other strand, where it starts forming the second strand. The next step is amplification. This can be done via PCR or IVT (in vitro transcription). PCR is nonlinear, resulting in bias because its efficiency is based on the sequence. IVT, though it is linear, produces a stronger 3’ coverage bias because the process itself...
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