Why was the reverse transcriptase used?

Tutor's Answer

(Top Tutor) Studyfaq Tutor
Reverse transcriptase was used to convert RNA to complementary DNA (cDNA) which was used as a template in the qRT-PCR reaction for detection of gene expression products. Brilliant II SYBR Green PCR Master Mix provided all the components and reagents needed for the reaction.  Careful primer design was critical to prevent undesired primer complementarity which could affect the accuracy of results. DNA was amplified using the same three steps as standard PCR: denaturation; annealing; and...
Completed Work